| 1. | Glass . glassware for bacteriological use . culture flask called " legroux tube "
玻璃.实验室玻璃器皿. “李柯氏管”培养瓶 |
| 2. | The viable cells after counting with trypan blue dye exclusion were then transferred to culture flask containing dmem medium in a density of 1 10 ^ 6
方法无菌条件下，从6月龄新西兰白兔的膝关节囊内剪取滑膜组织，采用组织块培养法和酶消化法分离滑膜细胞。 |
| 3. | Cartesian coordinates robotic system was developed to transplant tissue culture plantlets or scions from a flat plane to culture flasks in order to minimize the need of labor
摘要本研究设置一套直角座标机器人系统，用以扦?移植组织培养苗至培养瓶或相关容器中，期能替换移植作业的人工，降低此一产业的人力需求。 |
| 4. | Cartesian coordinates robotic system was developed to transplant tissue culture plantlets or scions from a flat plane to culture flasks in order to minimize the need of labor
摘要本研究设置一套直角座标机器人系统，用以扦?移植组织培养苗至培养瓶或相关容器中，期能取代移植作业的人工，降低此一产业的人力需求。 |
| 5. | A cartesian coordinates robotic system was developed to transplant tissue culture plantlets or scions from a flat plane to culture flasks in order to minimize the need of labor
摘要本研究设置一套直角座标机器人系统，用以扦?移植组织培养苗至培养瓶或相关容器中，期能替换移植作业的人工，降低此一产业的人力需求。 |
| 6. | Results obtained included : ( 1 ) mscs began to attach to culture flask after 24h cell inoculation by centrifugation method . cell proliferation was active after 3d . and mscs began to attach to culture flask only after 36h cell inoculation by erythrocyte splitting method
研究结果显示： （ 1 ）密度梯度高心法接种后24小时可见少数细胞贴壁， 3天后，细胞增殖活跃。 |
| 7. | In this experiment , radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process . methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0 . 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al . thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum , and added to 50ml cell culture flasks
方法：胰岛的分离参照王玲等的方法，每次实验取新生3 - 5天sd大鼠，无菌条件下剖腹取出胰腺，剪切为0 . 5mm ~ 3的组织块， v型胶原酶消化30min后，离心洗涤，悬浮于完全培养基，接种入50ml培养瓶，于5 co _ 2 、 95空气条件下培养20h ，转板纯化，接种于96孔培养板培养24h ，按实验要求进行实验。 |
| 8. | The results of the experimental tests show that nh4cl and k2hpo4 , kh2po4 are the most available nutrents to our isolates when they are degrading oils . the most suitable experinment condition is : temperature being 30 c , initial ph being 7 - 8 , the shaking rate of the culture flasks is 180 r / min , the concentration of nacl is 1 % . the biodegradation results have shown that our 6 isolates have the effective degradation capability to crude oil ; within 6 days the transforing rate of oil by each single strain is more than 60 %
本研究分离出的6株菌，初步鉴定结果为： sy1为微杆菌属、 sy2为诺卡氏菌属、 sy3和sy5为假单胞菌属、 sy4和sy6为芽孢杆菌属；实验结果表明， 6菌株的最佳氮源为氯化铵( nh _ 4cl ) ，最佳磷源为磷酸氢二钾和磷酸二氢钾的混合物，最适生长条件为：温度为30 ，初始ph值为7 8 ，摇床转速为180r min ，盐( nacl )浓度为1 ；通过降解实验得出6株菌对原油都有较强的降解能力，单一菌株在5天后的原油降解率都高于60 ， 6株菌对原油的生物降解反应符合一级反应动力学特征。 |
